Masterclass Webinar 2: Advanced Techniques and Workflows for Multiplex IHC Analysis using HALO® and HALO AI

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Following up on our Masterclass Webinar 1 for Multiplex IHC analysis, Masterclass 2 will discuss advanced workflows to help users tackle more challenging brightfield multiplex IHC assays. First, we will demonstrate how advanced cell segmentation and phenotyping options using HALO AI can be combined with the multiplex IHC module to improve image analysis. Two alternative approaches for analyzing multiplex IHC will also be demonstrated including a serial stain workflow to convert brightfield images into pseudo-fluorescence images followed by analysis with Highplex FL and a HALO AI-based approach where cell phenotyping is used to identify cells with co-localization of specific stains. We will discuss advantages, disadvantages and use cases for each workflow.

Learning Objectives:

  1. Advance your multiplex IHC module analysis with HALO AI cell segmentation and phenotyping options 
  2. Learn how to perform a serial stain image analysis workflow to convert multiplexed immunohistochemistry to pseudo-fluorescent images
  3. Learn advantages of AI-based phenotyping and how to perform it with HALO AI