HALO® | Image Analysis Platform

HALO Video Tutorials
21 Topics
01. OPEN & MANAGE IMAGES
02. NAVIGATE IMAGES
03. IMAGE ORIENTATION
04. BASIC ANNOTATION TOOLS
05. ANNOTATION LAYERS
06. ADVANCED ANNOTATIONS
07. ANNOTATIONS MANAGER
08. IMAGE REGISTRATION
09. VIEW SETTINGS AND CHANNEL RENAME
10. FIGURE MAKER
11. SELECT ANALYSIS MODULES | SETTINGS
12. SAVE CUSTOMIZED ANALYSIS SETTINGS
13. LOAD CUSTOMIZED ANALYSIS SETTINGS
14. ANALYZE A BATCH OF IMAGES
15. EXPORT ANALYSIS RESULTS FOR A SINGLE IMAGE
16. EXPORT ANALYSIS RESULTS FOR MULTIPLE IMAGES
17. ADVANCED EXPORT MANAGER 3.3
18. CREATE HISTOGRAMS
19. CREATE PLOTS | HEATMAPS
20. CROP TOOL
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HALO Webinars
24 Topics
Masterclass Webinar 2: Advanced Techniques and Workflows for Multiplex IHC Analysis using HALO® and HALO AI
Masterclass Webinar 1: Optimizing a 3-plex Multiplex IHC Assay Using Roche Discovery Reagents and HALO® Image Analysis
Getting Started with RNAscope™ Image Analysis in HALO®
HALO and HALO AI 3.6 Sneak Peek
HALO and HALO Link 3.6 Sneak Peek
Quantitative Digital Pathology: An Overview of the HALO® Image Analysis Platform
Masterclass Webinar: Neurobiology Image Analysis with HALO® and HALO AI
HALO and HALO AI 3.5 Sneak Peek
2022 Masterclass: Optimizing RNAscope Image Analysis
HALO, HALO AI, and HALO Link 3.4 Sneak Peek
2021 Masterclass: Approaches to Analyzing Skin Tissue using HALO and HALO AI
2021 Masterclass: Indica Labs Tech Toolbox
2021 Masterclass: Approaches to Kidney Tissue Analysis Using HALO and HALO AI
2021 Masterclass: Mastering RNA-protein Codetection Analysis in HALO
2021 Masterclass: Approaches to Pancreatic Tissue Analysis Using HALO and HALO AI
2021 Masterclass: Object-Based Analysis
2020 Masterclass – Applications in Toxicologic Pathology
2020 Masterclass – HALO Tips and Tricks
2021 Masterclass: Tips & Tricks Batch Processing Productivity Tools
HALO and HALO AI 3.2 Sneak Peek
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Masterclass Webinar 1: Optimizing a 3-plex Multiplex IHC Assay Using Roche Discovery Reagents and HALO® Image Analysis

HALO® | Image Analysis Platform HALO Webinars Masterclass Webinar 1: Optimizing a 3-plex Multiplex IHC Assay Using Roche Discovery Reagents and HALO® Image Analysis

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In this 60-minute webinar, we will discuss best practices and optimization techniques for Roche Discovery multiplex immunohistochemical (mIHC) assays and HALO® image analysis. First, we will present methods, tips and tricks to design and optimize staining protocols. Next, we will share a series of mIHC assays where various chromogen combinations were tested on an assay comprised of three biomarkers and hematoxylin. From this dataset, we will discuss which assays are appropriate for image analysis with the HALO® Multiplex IHC module, which are not, and why.

Learning Objectives:

  1. Advance your mIHC assay design and optimization knowledge 
  2. Improve your brightfield image deconvolution for mIHC analysis with tips and tricks 
  3. Learn from examples of successful 3-plex chromogenic image analysis as well as images that are problematic and learn from common challenges
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