In this 60-minute webinar, we will discuss best practices and optimization techniques for Roche Discovery multiplex immunohistochemical (mIHC) assays and HALO® image analysis. First, we will present methods, tips and tricks to design and optimize staining protocols. Next, we will share a series of mIHC assays where various chromogen combinations were tested on an assay comprised of three biomarkers and hematoxylin. From this dataset, we will discuss which